產(chǎn)品(pǐn)中心(xīn)
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FITC標(biāo)記的(dí)二磷酸腺苷(gān)核糖基(jī)化因子鳥(niǎo)嘌呤核(hé)苷(gān)酸交換因(yīn)子2抗(kàng)體(tǐ)
英(yīng)文(wén)名稱(chēng)Anti-ARFGEF2/FITC
中(zhōng)文名(míng)稱:FITC標記的二磷(lín)酸(suān)腺苷(gān)核糖基化因(yīn)子(zǐ)鳥(niǎo)嘌呤核(hé)苷酸(suān)交(jiāo)換(huàn)因(yīn)子2抗體
別 名ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited); ADP ribosylation factor guanine nucleotide exchange factor 2; ARFGEF 2; ARFGEF2; ARFGEP2; BIG 2; BIG2; Brefeldin A inhibited 2; Brefeldin A inhibited GEP 2; Brefeldin A inhibited guanine nucleotide exchange protein 2; dJ1164I10.1; BIG2_HUMAN.
規格:100ul
說 明 書100ul
研究(jiū)領(lǐng)域(yù)細胞生物 神(shén)經生物(wù)學(xué) 信號轉導(dǎo) G蛋白信號(hào)
抗體(tǐ)來源(yuán)Rabbit
克隆類(lèi)型(xíng)Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
產(chǎn)品(pǐn)應用ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分(fēn) 子 量(liáng)202kDa
細胞定(dìng)位(wèi)細(xì)胞膜
性 狀(zhuàng)Lyophilized or Liquid
濃 度(dù)1mg/ml
免(miǎn) 疫 原KLH conjugated synthetic peptide derived from human ARFGEF2/BIG2
亞(yà) 型(xíng)IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件(jiàn)Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介(jiè)紹background:
Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects golgi structure, and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways
Function:
ATBF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATBF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness.
Subunit:
Interacts with FNBP3 (By similarity). Interacts with PIAS3.
Subcellular Location:
Nuclear
Tissue Specificity:
Not found in normal gastric mucosa but found in gastric carcinoma cells (at protein level).
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 22 C2H2-type zinc fingers.
Contains 4 homeobox DNA-binding domains.
中(zhōng)文名(míng)稱:FITC標記的二磷(lín)酸(suān)腺苷(gān)核糖基化因(yīn)子(zǐ)鳥(niǎo)嘌呤核(hé)苷酸(suān)交(jiāo)換(huàn)因(yīn)子2抗體
別 名ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited); ADP ribosylation factor guanine nucleotide exchange factor 2; ARFGEF 2; ARFGEF2; ARFGEP2; BIG 2; BIG2; Brefeldin A inhibited 2; Brefeldin A inhibited GEP 2; Brefeldin A inhibited guanine nucleotide exchange protein 2; dJ1164I10.1; BIG2_HUMAN.
詳(xiáng)細介紹：
規格:100ul
說 明 書100ul
研究(jiū)領(lǐng)域(yù)細胞生物 神(shén)經生物(wù)學(xué) 信號轉導(dǎo) G蛋白信號(hào)
抗體(tǐ)來源(yuán)Rabbit
克隆類(lèi)型(xíng)Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
產(chǎn)品(pǐn)應用ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分(fēn) 子 量(liáng)202kDa
細胞定(dìng)位(wèi)細(xì)胞膜
性 狀(zhuàng)Lyophilized or Liquid
濃 度(dù)1mg/ml
免(miǎn) 疫 原KLH conjugated synthetic peptide derived from human ARFGEF2/BIG2
亞(yà) 型(xíng)IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件(jiàn)Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
相(xiāng)關資料：
產(chǎn)品介(jiè)紹background:
Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects golgi structure, and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways
Function:
ATBF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATBF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness.
Subunit:
Interacts with FNBP3 (By similarity). Interacts with PIAS3.
Subcellular Location:
Nuclear
Tissue Specificity:
Not found in normal gastric mucosa but found in gastric carcinoma cells (at protein level).
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 22 C2H2-type zinc fingers.
Contains 4 homeobox DNA-binding domains.
