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英文(wén)名(míng)稱 Anti-phospho-Bim(Ser87)
中文名稱(chēng)： 磷酸化(huà)細胞死亡(wáng)調解子抗體
別 名(míng) BCL2 like 11; Apoptosis facilitator;BAM; Bcl 2 interacting mediator of cell death; Bcl 2 interacting protein Bim; Bcl 2 like protein 11; Bcl 2 related ovarian death agonis; Bcl 2 related ovarian death agonist; BCL2 interacting mediator of cell death; BCL2 interacting protein Bim; BCL2 like 11 (apoptosis facilitator); BCL2 like 11; BCL2 like 11 apoptosis facilitator; BCL2 like protein 11; BCL2 related ovarian death agonist; BCL2L11; BIM alpha3; BIM alpha4; BIM alpha5; BIM alpha6; BIM; BIM beta6; BIM beta7; BimEL; BimL; BOD; B2L11_HUMAN.
濃 度 1mg/1ml
規 格 0.1ml/100μg
抗(kàng)體(tǐ)來源(yuán) Rabbit
克(kè)隆類型(xíng) polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
產品類(lèi)型 一(yī)抗 磷酸(suān)化抗(kàng)體(tǐ)
研究(jiū)領域(yù) 腫瘤 細(xì)胞生物(wù) 神經(jīng)生物學 信號轉(zhuǎn)導 細(xì)胞(bāo)凋亡 激酶(méi)和磷酸(suān)酶(méi)
蛋白分子(zǐ)量 predicted molecular weight: 22kDa
性 狀 Lyophilized or Liquid
免(miǎn) 疫(yì) 原 KLH conjugated Synthesised phosphopeptide derived from human Bim around the phosphorylation site of Ser87
亞 型 IgG
純(chún)化(huà)方法 affinity purified by Protein A
儲 存(cún) 液(yè) 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品(pǐn)應用(yòng) WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟(là)切片(piàn)需做(zuò)抗原(yuán)修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存(cún)條(tiáo)件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品(pǐn)介紹(shào) Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.
Function : Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit : Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK.
Subcellular Location : Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimEL: Mitochondrion. Isoform BimL: Mitochondrion. Isoform BimS: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.
Tissue Specificity : Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Similarity : Belongs to the Bcl-2 family.
Database links : UniProtKB/Swiss-Prot: O43521.1
Bim是Bcl-2家族BH3-only亞(yà)家族(zú)的(dí)成員,是一(yī)種(zhǒng)重(zhòng)要(yào)的(dí)凋(diāo)亡調節蛋白(bái)，具有助(zhù)凋(diāo)亡活性(xìng)作(zuò)用(yòng)。Bim廣(guǎng)泛(fàn)表達於正常細胞,對(duì)防御(yù)自身(shēn)免(miǎn)疫(yì)，維(wéi)持造血細胞穩(wěn)態有(yǒu)重要意(yì)義(yì),並(bìng)具有腫瘤(liú)抑製(zhì)因(yīn)子活性.Bim的生理作用(yòng)與(yǔ)它調節(jié)細胞(bāo)凋亡的(dí)功(gōng)能有(yǒu)關。
中文名稱(chēng)： 磷酸化(huà)細胞死亡(wáng)調解子抗體
別 名(míng) BCL2 like 11; Apoptosis facilitator;BAM; Bcl 2 interacting mediator of cell death; Bcl 2 interacting protein Bim; Bcl 2 like protein 11; Bcl 2 related ovarian death agonis; Bcl 2 related ovarian death agonist; BCL2 interacting mediator of cell death; BCL2 interacting protein Bim; BCL2 like 11 (apoptosis facilitator); BCL2 like 11; BCL2 like 11 apoptosis facilitator; BCL2 like protein 11; BCL2 related ovarian death agonist; BCL2L11; BIM alpha3; BIM alpha4; BIM alpha5; BIM alpha6; BIM; BIM beta6; BIM beta7; BimEL; BimL; BOD; B2L11_HUMAN.
詳(xiáng)細介(jiè)紹(shào)：
濃 度 1mg/1ml
規 格 0.1ml/100μg
抗(kàng)體(tǐ)來源(yuán) Rabbit
克(kè)隆類型(xíng) polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
產品類(lèi)型 一(yī)抗 磷酸(suān)化抗(kàng)體(tǐ)
研究(jiū)領域(yù) 腫瘤 細(xì)胞生物(wù) 神經(jīng)生物學 信號轉(zhuǎn)導 細(xì)胞(bāo)凋亡 激酶(méi)和磷酸(suān)酶(méi)
蛋白分子(zǐ)量 predicted molecular weight: 22kDa
性 狀 Lyophilized or Liquid
免(miǎn) 疫(yì) 原 KLH conjugated Synthesised phosphopeptide derived from human Bim around the phosphorylation site of Ser87
亞 型 IgG
純(chún)化(huà)方法 affinity purified by Protein A
儲 存(cún) 液(yè) 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品(pǐn)應用(yòng) WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟(là)切片(piàn)需做(zuò)抗原(yuán)修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存(cún)條(tiáo)件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
相(xiāng)關資(zī)料：
產品(pǐn)介紹(shào) Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.
Function : Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit : Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK.
Subcellular Location : Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimEL: Mitochondrion. Isoform BimL: Mitochondrion. Isoform BimS: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.
Tissue Specificity : Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Similarity : Belongs to the Bcl-2 family.
Database links : UniProtKB/Swiss-Prot: O43521.1
Bim是Bcl-2家族BH3-only亞(yà)家族(zú)的(dí)成員,是一(yī)種(zhǒng)重(zhòng)要(yào)的(dí)凋(diāo)亡調節蛋白(bái)，具有助(zhù)凋(diāo)亡活性(xìng)作(zuò)用(yòng)。Bim廣(guǎng)泛(fàn)表達於正常細胞,對(duì)防御(yù)自身(shēn)免(miǎn)疫(yì)，維(wéi)持造血細胞穩(wěn)態有(yǒu)重要意(yì)義(yì),並(bìng)具有腫瘤(liú)抑製(zhì)因(yīn)子活性.Bim的生理作用(yòng)與(yǔ)它調節(jié)細胞(bāo)凋亡的(dí)功(gōng)能有(yǒu)關。
