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一抗(kàng)
英(yīng)文名稱(chēng) Anti-Cyclin E
中(zhōng)文(wén)名稱(chēng) 周(zhōu)期素E抗體
別 名 CCNE 1; CCNE;CCNE1;Cyclin E1 antibody Cyclin Es;Cyclin Et;CyclinE; G1/S specific cyclin E; CCNE1_HUMAN.
詳(xiáng)細介紹：
濃(nóng) 度(dù) 1mg/1ml
規(guī) 格(gé) 0.2ml/200μg
抗(kàng)體(tǐ)來源(yuán) Rabbit
克隆類型 polyclonal
交(jiāo)叉(chā)反應 Human
產品類(lèi)型 一(yī)抗
研(yán)究領(lǐng)域 腫瘤(liú) 細胞生(shēng)物 神經生(shēng)物學 細胞凋(diāo)亡 細胞(bāo)周期蛋白 新陳(chén)代謝
蛋(dàn)白(bái)分(fēn)子(zǐ)量 predicted molecular weight: 47kDa
性 狀 Lyophilized or Liquid
免(miǎn) 疫(yì) 原 KLH conjugated synthetic peptide derived from human Cyclin E
亞(yà) 型 IgG
純化方(fāng)法 affinity purified by Protein A
儲 存(cún) 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產品(pǐn)應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需(xū)做(zuò)抗原修(xiū)復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保(bǎo)存條(tiáo)件(jiàn) Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
相關(guān)資料(liào)：
產(chǎn)品介紹(shào) Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Function : Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit : Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.
Subcellular Location : Nucleus.
Tissue Specificity : Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Post-translational modifications : Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Belongs to the cyclin family. Cyclin E subfamily.
細(xì)胞周期素E是(shì)調控細(xì)胞(bāo)G-1→S期(qī)轉(zhuǎn)變的關(guān)鍵因(yīn)素。由於(yú)在多種(zhǒng)腫(zhǒng)瘤中(zhōng)的(dí)不適(shì)當表(biǎo)達,細(xì)胞(bāo)周期(qī)素E現(xiàn)在(zài)已明確為原癌(ái)基因。
